Technical Physics Letters
To authors
Volumes and Issues
- 2026
- 2025
- 2024
- 2023
- 2022
Increased fibroblast stiffness as a result of pharmacological activation of mechanosensitive Piezo1 ion channels
Khalisov M. M.
1, Penniyaynen V. A.
1, Berintseva A. V.
1, Podzorova S. A.
1, Zegrya G. G.
1,2, Krylov B. V.
1
1Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia
2Ioffe Institute, St. Petersburg, Russia
2Ioffe Institute, St. Petersburg, Russia
Email: khalisovmm@infran.ru, penniyaynenv@infran.ru, berintsevaav@infran.ru, podzorova@infran.ru, krylov@infran.ru
The effects on cardiac fibroblasts of Jedi2 which is a pharmacological activator of mechanosensitive Piezo1 ion channels were studied. Using atomic force microscopy, it was found that Jedi2 at a concentration of 10 μM increases the cell stiffness. Immunofluorescence analysis showed an increase in the F-actin fluorescence intensity in cells at the above-specified concentration of Jedi2. Apparently, Jedi2 at a concentration of 10 μM activates Piezo1-mediated Ca2+-dependent signaling cascades, which elevates the F-actin level and, hence, leads to an increase in the cell stiffness. Keywords: Piezo1, Jedi2, fibroblasts, actin, atomic force microscopy.
- Nobel Prize in Physiology or Medicine 2021. [Electronic source]. https://www.nobelprize.org/prizes/medicine/2021/summary/
- B. Coste, J. Mathur, M. Schmidt, T.J. Earley, S. Ranade, M.J. Petrus, A.E. Dubin, A. Patapoutian, Science, 330 (6000), 55 (2010). DOI: 10.1126/science.1193270
- N.M. Blythe, K. Muraki, M.J. Ludlow, V. Stylianidis, H.T.J. Gilbert, E.L. Evans, K. Cuthbertson, R. Foster, J. Swift, J. Li, M.J. Drinkhill, F.A. van Nieuwenhoven, K.E. Porter, D.J. Beech, N.A. Turner, J. Biol. Chem., 294 (46), 17395 (2019). DOI: 10.1074/jbc.ra119.009167
- M.M. Khalisov, A.V. Berintseva, S.A. Podzorova, B.V. Krylov, V.A. Penniyaynen, Integrativnaya fiziologiya, 5 (1), 50 (2024). DOI: 10.33910/2687-1270-2024-5-1-50-59 (in Russian)
- R. Emig, W. Knodt, M.J. Krussig, C.M. Zgierski-Johnston, O. Gorka, O. Grob, P. Kohl, U. Ravens, R. Peyronnet, Cells, 10 (3), 663 (2021). DOI: 10.3390/cells10030663
- Y. Wang, S. Chi, H. Guo, G. Li, L. Wang, Q. Zhao, Y. Rao, L. Zu, W. He, B. Xiao, Nat. Commun., 9 (1), 1300 (2018). DOI: 10.1038/s41467-018-03570-9
- M.M. Khalisov, V.A. Penniyaynen, S.A. Podzorova, K.I. Timoshchuk, A.V. Ankudinov, B.V. Krylov, Tech. Phys., 65 (11), 1853 (2020). DOI: 10.1134/S106378422011016X
- J.L. Hutter, J. Bechhoefer, Rev. Sci. Instrum., 64, 1868 (1993). DOI: 10.1063/1.1143970
- I.N. Sneddon, Int. J. Eng. Sci., 3 (1), 47 (1965). DOI: 10.1016/0020-7225(65)90019-4
- K.I. Timoshchuk, M.M. Khalisov, V.A. Penniyaynen, B.V. Krylov, A.V. Ankudinov, Tech. Phys. Lett., 45 (9), 947 (2019). DOI: 10.1134/S1063785019090293
- P.D. Garcia, R. Garcia, Biophys. J., 114, 2923 (2018). DOI: 10.1016/j.bpj.2018.05.012
- D. Nevcas, P. Klapetek, Open Phys., 10 (1), 181 (2011). DOI: 10.2478/s11534-011-0096-2
- V.I. Chubinskiy-Nadezhdin, V.Y. Vasileva, I.O. Vassilieva, A.V. Sudarikova, E.A. Morachevskaya, Y.A. Negulyaev, Biochem. Biophys. Res. Commun., 514 (1), 173 (2019). DOI: 10.1016/j.bbrc.2019.04.139
- S. Orrenius, B. Zhivotovsky, P. Nicotera, Nat. Rev. Mol. Cell Biol., 4 (7), 552 (2003). DOI: 10.1038/nrm1150