Technical Physics Letters
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Selecting a fluorescence signal detection mode for DNA melting genetic analysis
Bulyanitsa A. L.
1, Aldekeeva A.S.
1, Belov D.A.
1
1Institute for Analytical Instrumentation of the Russian Academy of Sciences, Saint Petersburg, Russia
Email: antbulyan@yandex.ru, belov.da@list.ru
Successful implementation of the high-resolution melting analysis (HRMA) requires precise provision of temperature conditions. The paper presents the results of numerical modeling of thermal processes in the thermal block of the detecting amplifier, as well as the corresponding analytical solutions in a spatially one-dimensional approximation and with a medium homogeneous in thermal diffusivity. Estimates of the temperature distribution on the detecting amplifier are given when implementing various heating modes in different temperature ranges. The results obtained will reduce the errors in determining the melting temperature, or make the necessary corrections for the systematic error, and can be applied in the development of new devices. Keywords: DNA melting, temperature field, fluorescence, modeling.
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