A new chemiluminescence-based system based on a DNA-nanomachine with peroxidase-like activity (PxDm), integrated into a compact closed device, is presented. This system enables the complete workflow - from introduction of the sample under study to obtaining the result. The system was developed for detection of 16S rRNA Escherichia coli PxDm demonstrated high versatility and the ability to successfully detect target sequences with high specificity in various sample types: synthetic single-stranded DNA, extracted RNA, and unpurified bacterial lysates. This indicates the system's promise for real-world applications, where samples often contain complex matrix components and preliminary purification is challenging. The system exhibited high selectivity, distinguishing the target microorganism without responding to non-target microorganisms. The ability to work with unpurified samples and without enzymatic steps reduces analysis time and enables integration into point-of-care (POC) express-diagnostic formats. The use of PxDm under such conditions opens new prospects for rapid and accurate monitoring of bacterial infections and food safety control. Keywords:nucleic acid detection, chemiluminescence, G-quadruplex, DNA-nanomachine, bacterial pathogens.