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Home » Optics and Spectroscopy » Year 2026, issue 5 » Article p. 428
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Non-invasive optical monitoring of lower limb ischemia model
Russian version
Bukova V. I.1, Kovalchuk A. M.2, Makarova I. V.1, Guryleva A. V.1, Doroshenko Yu. S.2, Machikhin A. S.1, Baklaushev V. P.2,3
1Scientific and Technological Center of Unique Instrumentation of the Russian Academy of Sciences, Moscow, Russia
2Federal Center of Brain Research and Neurotechnologies of the Federal Medical and Biological Agency, Moscow, Russia
3Federal Scientific and Clinical Center for Specialized Medical Service and Medical Technologies, Moscow, Russia
Email: bukova.vi@ntcup.ru
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A method of lower limb ischemia monitoring via photoplethysmography and videocapillaroscopy is proposed. Critical lower limb ischemia was modeled on lab rats via embolization of arteria femoralis and verified using magnetic resonance angiography and perfusion analysis. Experimental setup and a pipeline for investigation are proposed. Quantitative parameters for ischemic limb's condition assessment are proposed; the parameters represent changes in skin blood perfusion and capillary network density. A decrease of these parameters' values in ischemic limb is presented. Data acquisition parameters, as well as parameters that present significant differences before embolization and one day after, high sensitivity and specificity, are described. Acquired results compliment conventional methods of ischemia assessment with data on skin microcirculation. The approach proposed provides robust data acquisition, non-invasive measurements and easy-to-implement hardware and software. In the future it can become a highly demanded supplement to conventional methods of ischemia diagnosis and quantitative assessment, also during prolonged experiments involving various therapeutic scenarios. Keywords: critical lower limb ischemia, non-invasive diagnostics, photoplethysmography, videocapillaroscopy, digital image processing, model animals.
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