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A comparative study of fresh and lyophilised biomass of the bacterium Enterobacter cloacae K-7 by vibrational spectroscopy techniques
Russian version
Kamnev A. A. 1, Dyatlova Yu. A. 1, Klimin S. A.2, Tugarova A. V. 1
1 Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Federal Scientific Centre of the RAS, Saratov, Russia
2Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, Moscow, Russia
Email: a.a.kamnev@mail.ru, jdyatlowa2013@yandex.ru, klimin@isan.troitsk.ru, tugarova_anna@mail.ru
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A comparative analysis of Raman spectra of fresh (separated by centrifugation and washed from the culture medium; without drying) and lyophilised biomass samples of planktonic culture of the bacterium Enterobacter cloacae K-7, as well as of a Fourier transform infrared (FTIR) spectrum of the lyophilised biomass, was performed. It has been shown that for fresh biomass, the presence of a significant amount of water in the sample does not lead to masking or shifting the main bands of the functional groups of biomacromolecules characteristic of bacteria in the case of Raman spectra, compared to lyophilised E. cloacae cells. For the latter, both the Raman and FTIR spectra also contain vibrational bands of the main functional groups typical of bacteria. The results obtained indicate the possibility of using both methods of sample preparation of bacterial biomass samples for general comparative analysis of their biomacromolecular composition by Raman spectroscopy and, in the case of lyophilised biomass, by FTIR spectroscopy. Keywords: bacterial biomass, Raman spectroscopy, FTIR spectroscopy, lyophilization, Enterobacter cloacae.
  1. A. Kassem, L. Abbas, O. Coutinho, S. Opara, H. Najaf, D. Kasperek, K. Pokhrel, X. Li, S. Tiquia-Arashiro. Front. Microbiol., 14, 1304081 (2023). DOI: 10.3389/fmicb.2023.1304081
  2. A.A. Kamnev, A.V. Tugarova. J. Analyt. Chem., 78 (10), 1320 (2023). DOI: 10.1134/S1061934823100106
  3. G. Pezzotti. J. Raman Spectrosc., 52 (12), 2348-2443 (2021). DOI: 10.1002/jrs.6204
  4. K.S. Lee, Z. Landry, A. Athar, U. Alcolombri, P. Pramoj Na Ayutthaya, D. Berry et al. Nat. Microbiol., 9, 1152-1156 (2024). DOI: 10.1038/s41564-024-01656-3
  5. E. Fardelli, A. D'Arco, S. Lupi, D. Billi, R. Moeller, M. Cestelli Guidi. Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 288, 122148 (2023). DOI: 10.1016/j.saa.2022.122148
  6. I. Saraeva, D. Zayarny, E. Tolordava, A. Nastulyavichus, R. Khmelnitsky, D. Khmelenin, S. Shelygina, S. Kudryashov. Chemosensors, 11 (7), 361 (2023). DOI: 10.3390/chemosensors11070361
  7. C. Lima, H. Muhamadali, R. Goodacre. Sensors, 22 (10), 3928 (2022). DOI: 10.3390/s22103928
  8. A.V. Tugarova, P.V. Mamchenkova, Yu.A. Dyatlova, A.A. Kamnev. Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 192, 458-463 (2018). DOI: 10.1016/j.saa.2017.11.050
  9. M.G. Fernandez-Manteca, A.A. Ocampo-Sosa, D.F. Vecilla, M.S. Ruiz, M.P. Roiz, F. Madrazo, J. Rodri guez-Grande, J. Calvo-Montes, L. Rodri guez-Cobo, J.M. Lopez-Higuera, M.C. Farinas, A. Cobo. Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 319, 124533 (2024). DOI: 10.1016/j.saa.2024.124533
  10. R.T. Vulchi, V. Morgunov, R. Junjuri, T. Bocklitz. Molecules, 29 (19), 4748 (2024). DOI: 10.3390/molecules29194748
  11. A.A. Kamnev, A.V. Tugarova, Yu.A. Dyatlova, P.A. Tarantilis, O.P. Grigoryeva, A.M. Fainleib, S. De Luca. Spectrochim. Acta Part A: Mol. Biomol. Spectrosc., 193, 558-564 (2018). DOI: 10.1016/j.saa.2017.12.051
  12. A.A. Kamnev, Yu.A. Dyatlova, O.A. Kenzhegulov, A.A. Vladimirova, P.V. Mamchenkova, A.V. Tugarova. Molecules, 26 (4), 1146 (2021). DOI: 10.3390/molecules26041146
  13. A.A. Kamnev, Yu.A. Dyatlova, O.A. Kenzhegulov, Yu.P. Fedonenko, S.S. Evstigneeva, A.V. Tugarova. Molecules, 28 (4), 1949 (2023). DOI: 10.3390/molecules28041949
  14. M.Z. Iqbal, K. Singh, R. Chandra. Cleaner Eng. Technol., 23, 100845 (2024). DOI: 10.1016/j.clet.2024.100845
  15. R. Singh, S. Kaur, S.S. Bhullar, H. Singh, L.K. Sharma. J. Sustain. Agric. Environ., 3, e12085 (2024). DOI: 10.1002/sae2.12085
  16. N. Khan, S. Ali, M.A. Shahid, A. Mustafa, R.Z. Sayyed, J.A. Cura. Cells, 10 (6), 1551 (2021). DOI: 10.3390/cells10061551
  17. B.N. Aloo, V. Tripathi, B.A. Makumba, E.R. Mbega. Front. Plant Sci., 13, 1002448 (2022). DOI: 10.3389/fpls.2022.1002448
  18. A.A. Agboola, T.A. Ogunnusi, O.G. Dayo-Olagbende, O.B. Akpor. Open Microbiol. J., 17 (1), e187428582308080 (2023). DOI: 10.2174/18742858-v17-230823-2023-8
  19. R.P. Singh, D.M. Pandey, P.N. Jha, Y. Ma. PloS One, 17 (5), e0267127 (2022). DOI: 10.1371/journal.pone.0267127
  20. Ye.V. Kryuchkova, G.L. Burygin, N.E. Gogoleva, Yu.V. Gogolev, M.P. Chernyshova, O.E. Makarov, E.E. Fedorov, O.V. Turkovskaya. Microbiol. Res., 169, 99-105 (2014). DOI: 10.1016/j.micres.2013.03.002
  21. K.K. Meena, N.K. Taneja, A. Ojha, S. Meena. Ann. Phytomed., 12 (1), 706-716 (2023). DOI: 10.54085/ap.2023.12.1.76
  22. C.A. Morgan, N. Herman, P.A. White, G. Vesey. J. Microbiol. Meth., 66 (2), 183-193 (2006). DOI: 10.1016/j.mimet.2006.02.017
  23. A.H. Garci a. J. Biosci., 36 (5), 939-950 (2011). DOI: 10.1007/s12038-011-9107-0
  24. J.T. Lennon, S.E. Jones. Nat. Rev. Microbiol., 9, 119-130 (2011). DOI: 10.1038/nrmicro2504
  25. A.J. Szekely, S. Langenheder. ISME J., 11, 1764-1776 (2017). DOI: 10.1038/ismej.2017.55
  26. E.T. da Cunha, A.M. Pedrolo, A.C.M. Arisi. Arch. Microbiol., 205 (5), 190 (2023). DOI: 10.1007/s00203-023-03542-8
  27. S. Mahmud, S. Khan, M.R. Khan, J. Islam, U.K. Sarker, G.M.M.A. Hasan, M. Ahmed. J. Food Proc. Preserv., 46, e17123 (2022). DOI: 10.1111/jfpp.17123
  28. O.A. Kenzhegulov, Yu.A. Dyatlova, S.A. Klimin, A.V. Tugarova, A.A. Kamnev. Microbiology (Moscow), 93 (Suppl. 1), S153-S156 (2024). DOI: 10.1134/S0026261724609692
  29. M.L. Paret, S.K. Sharma, L.M. Green, A.M. Alvarez. Appl. Spectrosc., 64 (4), 433-441 (2010)
  30. F. SantAna de Siqueira e Oliveira, H.E. Giana, L. Silveira, Jr. J. Biomed. Optics, 17 (10), 107004 (2012). DOI: 10.1117/1.JBO.17.10.107004
  31. A. Nakar, A. Wagenhaus, P. Rösch, J. Popp. Analyst, 147, 3938-3946 (2022). DOI: 10.1039/d2an00822j
  32. J. Le Galudec, M. Dupoy, L. Duraffourg, V. Rebuffel, P.R. Marcoux. Microb. Biotechnol., 18, e70093 (2025). DOI: 10.1111/1751-7915.70093
  33. R.J. Delle-Bovi, A. Smits, H.M. Pylypiw. Amer. J. Anal. Chem., 2, 212-216 (2011). DOI: 10.4236/ajac.2011.22025
  34. S. Correa-Garci a, M. Bermudez-Moretti, A. Travo, G. Deleris, I. Forfar. Anal. Methods, 6, 1855-1861 (2014). DOI: 10.1039/C3AY42322K

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